Molecular eco-epidemiology of Paracoccidioides brasiliensis in road-killed mammals reveals Cerdocyon thous and Cuniculus paca as new hosts harboring this fungal pathogen
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DADOS DA PUBLICAÇÃO
Título
Molecular eco-epidemiology of Paracoccidioides brasiliensis in road-killed mammals reveals Cerdocyon thous and Cuniculus paca as new hosts harboring this fungal pathogen
Resumo
Wild animals infected with Paracoccidioides brasiliensis represent important indicators of
this fungal agent presence in the environment. The detection of this pathogen in road-killed
wild animals has shown to be a key strategy for eco-epidemiological surveillance of paracoccidioidomycosis
(PCM), helping to map hot spots for human infection. Molecular detection
of P. brasiliensis in wild animals from PCM outbreak areas has not been performed so far.
The authors investigated the presence of P. brasiliensis through nested-PCR in tissue samples
obtained from road-killed animals collected nearby a human PCM outbreak spot, Rio
de Janeiro state, Brazil and border areas. Eighteen species of mammals were analyzed:
Dasypus novemcinctus (nine-banded armadillo, n = 6), Cerdocyon thous (crab-eating fox, n
= 4), Coendou spinosus (hairy dwarf porcupine, n = 2), Lontra longicaudis (Neotropical river
otter, n = 1), Procyon cancrivorus (crab-eating raccoon, n = 1), Galactis cuja (lesser grison,
n = 1), Tamandua tetradactyla (collared anteater, n = 1), Cuniculus paca (paca, n = 1), and
Bradypus variegatus (brown-throated three-toed sloth, n = 1). Specific P. brasiliensis
sequences were detected in the liver, spleen, and lymph node samples from 4/6 (66.7%) D.
novemcinctus, reinforcing the importance of these animals on Paracoccidioides ecology.
Moreover, lymph nodes samples from two C. thous, as well as lung samples from the C.
paca were also positive. A literature review of Paracoccidioides spp. in vertebrates in Brazil
indicates C. thous and C. paca as new hosts for the fungal pathogen P. brasiliensis.
PLOS ONE
PLOS ONE
Autor(s) vinculado(s) à UVA
Coautor(s) externo(s)
Bruno de Souza Scramignon-Costa | Fernando Almeida-Silva | Bodo Wanke | Marcelo Weksler | Priscila Marques de Macedo
Ano de publicação
Revista / Periódico / Editora
PLOS ONE
Tipo de produção (selecione o tipo e subtipo)
Produção bibliográfica > Artigos completos publicados em periódicos
Palavras-chave
ODS relacionado
Citação completa
de Souza Scramignon-Costa B, Almeida-
Silva F, Wanke B, Weksler M, Moratelli R, do Valle
ACF, et al. (2021) Molecular eco-epidemiology of
Paracoccidioides brasiliensis in road-killed
mammals reveals Cerdocyon thous and Cuniculus
paca as new hosts harboring this fungal pathogen.
PLoS ONE 16(8): e0256668. https://doi.org/
10.1371/journal.pone.0256668
VINCULAÇÃO
Curso(s) vinculados
Linha de pesquisa
Conservação da Biodiversidade | Monitoramento epidemiológico